Dichotomous splicing signals in exon flanks

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Dichotomous splicing signals in exon flanks.

Intronic elements flanking the splice-site consensus sequences are thought to play a role in pre-mRNA splicing. However, the generality of this role, the catalog of effective sequences, and the mechanisms involved are still lacking. Using molecular genetic tests, we first showed that the approximately 50-nt intronic flanking sequences of exons beyond the splice-site consensus are generally impo...

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Evolutionary divergence of exon flanks: a dissection of mutability and selection.

The intronic sequences flanking exon-intron junctions (i.e., exon flanks) are important for splice site recognition and pre-mRNA splicing. Recent studies show a higher degree of sequence conservation at flanks of alternative exons, compared to flanks of constitutive exons. In this article we performed a detailed analysis on the evolutionary divergence of exon flanks between human and chimpanzee...

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Facilitation from collinear flanks is cancelled by non-collinear flanks

Detection of a central Gabor pattern is facilitated by the presence of collinear flanking patterns. We find that this facilitation is greatly reduced when the collinear flanks are combined with non-collinear flanks to form a coherent surround. These results are unlikely to be explained by mechanisms that merely transduce local contrast in a nonlinear fashion. A model wherein the outputs of such...

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hnRNP A1 recruited to an exon in vivo can function as an exon splicing silencer.

Some exons contain exon splicing silencers. Their activity is frequently balanced by that of splicing enhancers, and this is important to ensure correct relative levels of alternatively spliced mRNAs. Using an immunoprecipitation and UV-cross-linking assay, we show that RNA molecules containing splicing silencers from the human immunodeficiency virus type 1 tat exon 2 or the human fibroblast gr...

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Bioinformatic analysis of exon repetition, exon scrambling and trans-splicing in humans

MOTIVATION Using bioinformatic approaches we aimed to characterize poorly understood abnormalities in splicing known as exon scrambling, exon repetition and trans-splicing. RESULTS We developed a software package that allows large-scale comparison of all human expressed sequence tags (EST) sequences to the entire set of human gene sequences. Among 5,992,495 EST sequences, 401 cases of exon re...

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ژورنال

عنوان ژورنال: Genome Research

سال: 2005

ISSN: 1088-9051

DOI: 10.1101/gr.3217705